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pspgrna vector  (Addgene inc)


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    Addgene inc pspgrna vector
    Pspgrna Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pspgrna+vectors/pSPgRNA+(Plasmid+%2347108)/pm40010349-220-11-13
    Average 96 stars, based on 164 article reviews
    pspgrna vector - by Bioz Stars, 2026-09
    96/100 stars

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    Transfection:

    Article Title: CRISPR-Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta-Galactoside Alpha-2,6-Sialyltransferase 1 in CHO Cells.
    Article Snippet: gRNAs were designed by the CRISPR/Cas9 target online predictor (CCtop) [47] and cloned into the pSpgRNA vector (Addgene, #47108) according to the protocol published by Bauer et al. [48]. .. For targeted demethylation, plasmids pCAG-dCas9-5xPlat2AflD (Addgene, #82560) (from now on dCas9-GCN4) and pCAG-scFvGCN4sfGFPTET1CD (Addgene, #82561) (from now on scFv-TET1) were used in combination with pSPgRNA vectors. scFv-TET1 contains a superfolder GFP (sfGFP) in order to measure transfection efficiencies. .. Plasmids were amplified in E. coli cultures grown in Luria-Bertani broth and isolated with the EndoFree Plasmid Maxi Kit (Qiagen) and eluted in UltraPureTM DNase/RNase-Free Distilled Water (Thermo Fisher Scientific).



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